Activity-dependent enhancement of synaptic transmission in hippocampal slices treated with the phosphatase inhibitor calyculin A

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Activity-dependent enhancement of synaptic transmission in hippocampal slices treated with the phosphatase inhibitor calyculin A.

The role of protein phosphatases in regulating synaptic transmission in the CA1 region of the hippocampus was examined using slices pretreated with calyculin A, a specific inhibitor of protein phosphatases 1 and 2A. Stimulation of afferents at 1 Hz (but not 0.1 Hz) for periods of 5-10 min caused a long-lasting enhancement of synaptic transmission. The increase in synaptic responses was not due ...

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The effect of ketamine (1-100 µM), which has NMDA receptor antagonist properties, on synaptic transmission and long-term potentiation (LTP) in CAl area of rat hippocampus was examined in vitro. Field potentials were recorded in pyramidal cell layer following Schaffer collateral stimulation. Primed-burst stimulation (PEs) was used for induction of LTP. The amplitude of population spiks (PS) was ...

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Periodic fluctations in synaptic transmission and enhancement of transmission in hippocampal slices.

The properties of the synchronously activated radiatum fiber-CA1 synaptic population were examined with the in vitro hippocampal slice preparation. Periodic fluctuations in synaptic transmission and in the enhancement of synaptic transmission were observed with periods ranging from 8 to 20 s. Such periodic fluctuations did not arise from fluctuations in afferent radiatum fiber activity. The per...

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Improved posthypoxic recovery of synaptic transmission in gerbil neocortical slices treated with a calpain inhibitor.

BACKGROUND AND PURPOSE Among the various calcium-induced biologic events occurring in hypoxic neurons, activation of the calcium-activated neutral proteinase (calpain) is a likely mediator of neuronal degeneration. In this study, we assessed the protective effects of a calpain inhibitor (Cbz-Val-Phe-H) against hypoxic damage to the neocortex. METHODS An in vitro neocortical slice model from g...

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Enhancement of afferent fiber activity in hippocampal slices.

The potentiation of synaptic activity in the hippocampal formation is a well documented phenomenon. It has been suggested, however, that a recruitment of additional afferent fibers can contribute to such an increase in synaptic activity. The hypothesis of an enhancement in afferent fiber activity, therefore, was investigated with the in vitro hippocampal slice preparation. Isolated radiatum fib...

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ژورنال

عنوان ژورنال: The Journal of Neuroscience

سال: 1994

ISSN: 0270-6474,1529-2401

DOI: 10.1523/jneurosci.14-10-06013.1994